Pigment Stoichiometry of a Photosystem II Reaction Center Purified by a Single Step Perfusion Chromatography Procedure

Determining the pigment composition in the D 1/D2-cyt b559 complex of Photosystem II has become an important research objective during the last several years, because analysis of the early events in PSII RCs after pulsed illumination, is strongly dependent on the correlation between the optical absorption bands and particular pigments. Using various methods for isolating the complex, different groups have reported Chia to Pheo ratios of 4:2, 5:2 and 6:2 (1, 2, 3).