Determination of hyaluronic acid in the urine of a patient with Werner's syndrome.

A 54-year-old Japanese male with Werner's syndrome was reported. We found increased excretion of hyaluronic acid in the urine, although the content and analyses of the glycosaminoglycans in the urine eluted by Dowex-1 column chromatography was shown to be hyaluronic acid by comparison with standard hyaluronic acid. The Sephadex G-200 gel filtration of the 0.5 M NaCl fraction caused separation into two fractions. It was revealed that there was not only an excessive production of hyaluronic acid, but also an excretion of lower molecular weight hyaluronic acid in the urine of this syndrome.