Determination of Uric Acid in Human Serum: Reversed-Phase Liquid Chromatography with Electrochemical Detection

Abstract A method for the simultaneous determination of uric acid in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection has been developed. Human serum (0.5 ml) was mixed with 0.5 ml of 0.2 N perchloric acid solution and the mixture was centrifuged at 3,000 g for 20 min. An aliquot (10 μl) of the supernatant (deproteinized human serum) was injected into the chromatographic system employed in this study. The assay limit for quantitation was about 10 pg for uric acid. Complete separation of uric acid was achieved in about 8 min under the present chromatographic conditions.