Diurnal changes in leaflet gas exchange, water status and antioxidant responses in Carapa guianensis plants under water-deficit conditions

Young Carapa guianensis plants were examined under well-watered (control) and water-deficit conditions with the aim to evaluate possible relationship between diurnal changes in leaflet gas exchange with lipid peroxidation and adjustments in antioxidative responses. Treatment comparisons were assessed when leaflet water potential (Ψw) in water-stressed plants reached around −2.5 ± 0.5 MPa at pre-dawn. Regardless of watering regime, the highest net CO2 assimilation rate and stomatal conductance were recorded until 9:00 h. Control plants showed diurnal increases in transpiration, while it was strongly decreased in water-stressed plants. Diurnal decreases in intercellular to ambient CO2 concentration ratio were just observed in stressed plants. Regardless of watering regime, non-significant changes (P > 0.05) in Ψw and relative water content were registered throughout the day; however, both variables were significantly lower (P < 0.05) in stressed plants. Malondialdehyde concentration did not vary throughout the day, but it was higher in stressed plants. Excepting for guaiacol-type peroxidase, the antioxidant enzyme activities varied throughout the day regardless of watering regimes. Nevertheless, increases in antioxidant enzymes were more expressive in water-stressed plants. Despite, a relationship between diurnal changes in A and gs and lipid peroxidation or antioxidant enzymes was unclear regardless of watering regimes. Thus, we conclude that although plants from both watering regimes were able to adjust antioxidant enzymes activities throughout the day, the water-stressed plants were more susceptible to damages to net CO2 assimilation and suffered more expressive oxidative damages to lipids than plants grown under well-watered conditions.