Antiproliferative activity of individual herbs of Abnormal Savda Munziq on HL-60 cells.

Abnormal Savda Munziq (Patent no. C082130082.8) is a herbal formula composed of ten medicinal herbs,namely Glycyrrhiza uralensis,Ziziphus jujuba,Cordia dichotoma,Anchusa italica,Adiantum capillus-veneris,Euphorbia humifusa,Foeniculum vulgare,Lavandula angustifolia,Melissa officinalis and Alhagi pseudoalhagi. It is used in traditional Uighur medicine for the treatment and prevention of cancer,diabetes and cardiovascular diseases. This paper studies the antiproliferative activity of individual herbs of Abnormal Savda Munziq (except Alhagi pseudoalhagi) on HL-60 cells. Cell viability of HL-60 cells exposed to CH2Cl2 extract and MeOH extract of each herb was determined by the trypan blue exclusion method after 24 h,48 h and 72 h of incubation (37° C,5% CO2),respectively. As a result,except for Foeniculum vulgare,CH2Cl2 extracts of all herbs significantly reduce the viability of HL-60 cells (P0.05); MeOH extracts of only two herbs,namely Glycyrrhiza uralensis and Euphorbia humifusa,significantly reduce the viability of HL-60 cells (P0.05). The cell viability of cells exposed to CH2Cl2 extract of all herbs was shown to be significantly lower than that of cells exposed to its MeOH extract (P0.05). The results clearly show that the CH2Cl2 extracts of eight herbs,namely Cordia dichotoma,Euphorbia humifusa,Adiantum capillus-veneris,Glycyrrhiza uralensis,Ziziphus jujuba,Lavandula angustifolia,Anchusa italica,Melissa officinalis and the MeOH extracts of two herbs,namely Glycyrrhiza uralensis and Euphorbia humifusa,have in vitro antiproliferative activity on HL-60 cells. The antiproliferative activity of CH2Cl2 extract of each herb is stronger than that of its MeOH extract.