Investigation of arboviruses in different regions of Guizhou province,China in 2008

Objective To investigate the distribution profiles of arboviruses in different regions of Guizhou province,China.Methods Mosquito samples were collected using mosquito lamps in Qianxi county,Dejiang county,Rongjiang county,and Congjiang county,Guizhou province in July 2008 and then used for virus isolation by tissue culture.The obtained virus isolates were identified by serological and molecular biological methods.Homology and phylogenetic analysis were performed on viral sequences by bioinformatics.Results A total of 9160 mosquitoes(4 species,3 genera) were collected.Out of the 9 virus strains isolated from the mosquitoes,8 were identified as Getah virus(GETV),and 1 was identified as Japanese encephalitis virus(JEV).Compared with the prototype strain,the GETV strains had a nucleotide homology of 94.5%-94.9% and an amino acid homology of 97.4%-97.6%.The newly isolated JEV belonged to genotype Ⅰ,according to the homology and phylogenetic analysis.Conclusion GETV and genotype Ⅰ JEV were first isolated from mosquitoes collected in Guizhou province,China.The GETV strains in Guizhou province have a close phylogenetic association with the strains isolated from other provinces in China,and the JEV strain in Guizhou province has a close phylogenetic association with the strains isolated from Sichuan province,China.