A rapid detection procedure for the HtN gene under controlled inoculation of maize with Exserohilum turcicum

A technique for rapid and early detection of the HtN gene for resistance against Exserohilum turcicum, the cause of northern leaf blight, in maize (Zea mays) is based on quantitative inoculation (by air) with high inoculum pressure (100,000 and 200,000 conidia per square centimeter) on a leaf target, immediately followed by a long wetness period (48 hr). Under such optimal conditions for disease development, plants could be termed resistant or susceptible to E. turcicum as early as the five-leaf stage and 4-10 days after inoculation. In contrast to the commonly used drop inoculation, the technique suggested here does not allow the «escape» of susceptible plants as resistant. Currently, this technique is being used in our breeding program (.)