The method of probe design Dna microarray having a dna microarray with a probe designed by the method of

The present invention provides an excellent efficiency of detection of polymorphisms such as SNP microarray genomic DNA contained in DNA probes. A method of designing a probe according to the invention comprising: for genomic DNA derived from the subject organism contained restriction endonuclease recognition site endo sandwiched recognition restriction enzyme fragments of the specified covers at least part of the inner segment a plurality of steps or regions; specified one or more regions as probes for the detection of the test organism fragments to design step.