Recombinant human BMP-2 enhances osteogenesis of demineralized bone matrix in experimental mastoid obliteration

AbstractConclusions: From the results, recombinant human bone morphogenic protein 2 (rhBMP-2) activated demineralized bone matrix (DBM) for the enhancement of bone regeneration. These results might provide a basis for the clinical application of BMP-2 in mastoid obliteration. Objective: The purpose of this study was to evaluate the enhanced osteogenesis of rhBMP-2-loaded DBM using a gelatin sponge in the mastoid obliteration model. Methods: The bulla obliteration was done using rhBMP-2 (0.075 mg/ml)/DBM in experimental group I (n = 7) and rhBMP-2 (0.375 mg/ml)/DBM in experimental group II (n = 7). In the control group (n = 7), the bullae were obliterated using PBS/DBM. To assess the active mineralization of new bone formation, each group received intravenous calcein blue at 4 weeks, oxytetracycline hydrochloride at 8 weeks, and alizarin red at 10 weeks. The animals in each group were sacrificed 12 weeks post-surgery. Osteogenesis was evaluated by in vivo CT and histological observation. Results: The large...