Association of virions and coat protein of tobacco vein mottling potyvirus with cylindrical inclusions in tobacco cells

The course of infection with tobacco vein mottling potyvirus (TVMV) in tobacco protoplasts and leaves was studied by electron microscopy with immunogold labeling. In sections of protoplasts inoculated with TVMV RNA 10, 21, or 45 h prior to fixation, antibodies to TVMV coat protein (CP) were bound to cylindrical inclusions and individual virions usually associated with these inclusions. Also, in sections of leaves from plants inoculated with TVMV 5, 14, or 21 days prior to fixation, antibodies to TVMV CP were bound to cylindrical inclusions and aggregates of virions in the cytoplasm [...]