Influence of scopoletin on glutathione S-transferases in mesenteron of Bombyx mori Linnaeus.

【Objective】The influence of scopoletin on glutathione S-transferases(GSTs) in mesenteron of Bombyx mori Linnaeus was studied to provide references for mulberry field pest control and resistant line breeding of Bombyx mori Linnaeus. 【Method】This research adopted the leaf poisoning method to add poison to B. mori Linnaeus with scopoletin to find out the influence of scopoletin on GSTs in mesenteron of B. mori Linnaeus. IC50 of scopoletin on GSTs was detected via in vitro inhibition test. 【Result】B. mori Linnaeus showed similar toxic symptoms(spitting and anti-feeding) after eating mulberry leaves that were treated with different concentrations of scopoletin(0.026-0.104 mol/L). However, symptoms completely disappeared after 36 h. Compared with the normal silkworm, GSTs activity trends of midgut of silkworm fed with mulberry leaves treated by scopoletin went down in earlier stage, up in the middle stage and down again in late stage. Scopoletin under different concentrations could strikingly suppress the GSTs specific activity in mesenteron of B. mori Linnaeus. With the increase of concentration of scopoletin, the suppression ratio increased. IC50 of scopoletin on GSTs was 0.00474 mol/L. 【Conclusion】Scopoletin can repress GSTs activity in mesenteron of B. mori Linnaeus.