Study on cyfluthrin resistance and its mechanisms of Anopheles sinensis in Nanchang frontierport

: ［摘要］目的 了解南昌昌北国际机场中华按蚊的氟氯氰菊酯抗药性, 初步探索其产生机制。方法 应用WHO成蚊生 测法检测当地中华按蚊抗药性水平, 将生测过程中即将死亡蚊虫归为敏感蚊, 存活蚊虫归为抗性蚊, 检测并比较两组蚊 虫P450单加氧酶、谷胱甘肽巯基转移酶 (GST) 活性; 同时记录每只敏感蚊生测死亡时间, 对生测死亡时间与P450单加氧 酶、GST活性进行相关分析。结果 当地中华按蚊生测死亡率为59.5%; 抗性蚊、敏感蚊、实验室敏感蚊的P450单加氧酶 活性差异具有统计学意义 (F = 151.89, P 敏感蚊> 实验室敏感蚊; 但三者GST活性差异无统计学 意义 (F = 0.72, P＞0.05); 蚊虫生测死亡时间与P450单加氧酶活性呈正相关性, 相关系数为0.88, 回归方程为y = 79.479 + 1.512x; 生测死亡时间与GST活性不具有相关性。结论 南昌昌北国际机场中华按蚊对氟氯氰菊酯已产生抗药性, P450单加氧酶活性增强可能是其抗药性产生的机制之一。. METHODS: The resistance levels of the local An. sinensis were detected by WHO drug resistance bioassay. During the bioassay, the dying mosquitos were classed as sensitive mosquitos, and the survival ones were classed as resistant mosquitos. The P450 monooxygenase activity and glutathione S-transferase activity were detected and compared between the two groups. At the same time, the death time of each sensitive mosquito was recorded, and the correlations between the death time and the P450 monooxygenase activity and glutathione S-transferase activity were analyzed, respectively. RESULTS: The bioassay mortality of the local An. sinensis was 59.5%. The differences of the P450 monooxygenase activities among the resistant mosquitos, sensitive mosquitos and laboratory sensitive mosquitos had statistical significances (F =151.89, P sensitive mosquitos > laboratory sensitive mosquitos. The differences of glutathione s-transferase activities among the three groups had no statistical significance (F = 0.72, P = 0.49). There existed positive correlation between the mosquito death time and the P450 monooxygenase activity, and the regression equation was y = 79.479 +1.512x with the correlation coefficient of 0.88, while there was no correlation between the mosquito death time and the glutathione S-transferaseactivity. CONCLUSIONS: The An. sinensis in Nanchang Changbei International Airport has been resistant to cyfluthrin, and the promotion of P450 monooxygenase activity maybe one of the reasons for the resistance.