Synthesis of environmentally sensitive 2′-deoxyguanosine containing solvatochromic pyrene fluorophore

Abstract The synthesis and photophysical property of novel solvatochromic pyrene derivative, Apa 5 and fluorescent guanine Apa G 8 were described. These newly synthesized fluorescent pyrene derivatives exhibited solvent polarity dependent fluorescence at longer wavelengths. Such environmentally sensitive pyrene derivatives can be used as a reporter probe that is sensitive to the changes in the microenvironment around DNA either in vitro or in vivo.