Pharmacological Study on the Effects of Some Ocular Hypotensive Drugs on Aqueous Humor Formation in the Arterially Perfused Enucleated Rabbit Eye

The effects of pilocarpine, isoproterenol, epinephrine, timolol, acetazolamide, ouabain, and dinitrophenol on aqueous humor production were investigated in enucleated rabbit eye. The metabolic inhibitors ouabain and dinitrophenol at a concentration of 10––5M produced a 50% inhibition in aqueous humor formation. Acetazolamide at the same concentration was without effect, but at 10––4M concentration lowered aqueous production by 40%. The autonomic drugs epinephrine and isoproteranol at a concentration of 10 µg/ml medium reduced aqueous humor formation by 64 and 52% respectively. Timolol, even at a concentration of 0.05 µg/ml, suppressed aqueous humor formation by approximately 30%. Pilocarpine on the other hand was ineffective. Except for pilocarpine, these responses are similar to those reported to occur in the cat both in vivo and in vitro.