Interleukin‐1β Regulation of Adhesion Molecules on Human Gingival and Periodontal Ligament Fibroblasts

Background: Adhesion molecules have been implicated in the pathogenesis of rheumatoid arthritis and may also play a role in the pathogenesis of periodontal disease by promoting the recruitment and retention of leukocytes in gingival tissue. Methods: The aim of the present study was to evaluate the capacity of interleukin-1β (IL-1β) to regulate adhesion molecule expression on clinically healthy human gingival (HGF) and periodontal ligament (PDL) fibroblasts. The HGF (n = 6) and PDL (n = 3) fibroblasts were treated with 1.0 ng/ml of IL-1β for 24 hours and then incubated with primary intercellular adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) antibodies followed by FITC-conjugated secondary antibodies. The expression of ICAM-1 and VCAM-1 was measured by immunofluorescence flow cytometry. Results: The levels of ICAM-1 expression in IL-1β treated HGF and PDL fibroblasts were statistically significant (P ≤0.05) compared to normal untreated controls using log-transformed data an...