Inheritance of recombinant carp β-actin/GH cDNA gene in transgenic carp
1995
Abstract Individual carp ( Cyprinus carpio ) raised from zygotes that were microinjected with two different carp β-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males and females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as well as with non-transgenic carp. The level of transgene transmittance from the transgenic parents varied between individuals. We observed 32%–87% inheritance when transgenic parents were crossed and 0%–50% inheritance when transgenic parents were mated with non-transgenics.
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