Carbon tetrachloride does not induce micronucleus in either mouse bone marrow or peripheral blood

Abstract We performed mouse bone marrow and peripheral blood micronucleus tests on carbon tetrachloride (CCl 4 ). In the bone marrow assay, bone marrow cells were collected once after 24 h, and twice, with a 24-h interval at a dose of 500, 1000 and 2000 mg/kg. In the peripheral blood assay, blood samples were collected 0, 24, 48 and 72 h after a single intraperitoneal injection at a dose of 1000, 2000 and 3000 mg/kg. As a result, micronucleated polychromatic erythrocytes (MNPCEs) were observed neither in the bone marrow assay nor the peripheral blood assay. We concluded that CCl 4 does not induce chromosomal aberrations in the mouse bone marrow cells under these experimental conditions.