Local despiralization of DNA in lymphocytes of patients with chronic lympholeukemia

: DNA isolated from blood lymphocytes of 43 patients with chronic lymphoid leukemia was studied for the content of local despiralized sites by the kinetic formaldehyde method. The patients were classified into three groups: before, during and after chemotherapy by cytostatic drugs (cyclophosphamide or chlorambucil). It is found that DNA from leukosis lymphocytes contain more despiralized sites as against to DNA from normal lymphocytes and that after chemotherapy a degree of DNA despiralization decreases.