Production and evaluation of doubled haploid lines of barley via detached-tiller culture method

This research was conducted to compare the classic (C) and detached-tiller methods (a: sterile by clip and scissors D 1 ; b: sterile by hot water D 2 ) for producing haploids in barley. The parental materials used in this research were F 1 four genotypes of Hordeum vulgare: B 1 : Reihane × Legia; B 2 : Reihan × Igri; B 3 : Kavir × Igri; B 4 : Kavir × Legia, and the Hordeum bulbosum was PB1 genotype (Plant Breeding Institute). The traits such as seed set percentage, embryo development, haploid seedling development and produced doubled haploid lines were analyzed statically using χ 2 test. The comparison was made among arable barley B 1 , B 2 , B 3 , B 4 crossing wild barley of H. bulbosum . In C approach, there was no significant difference concerning the percent of forming seed, embryo and haploid production. While in D 1 approach, there was a significant difference in embryo forming percent but no significant difference was observed for haploid plant and seed set percent. Moreover, in D 2 approach, there was a significant difference for seed set percent. However, for haploid production and embryo development percent, the approach D 1 in regards with the percent of forming embryo and production of haploids was superior to the other two approaches, C and D 2 . Key words : Detached-tiller culture, haploid, doubled haploid, Hordeum bulbosum, Hordeum vulgare.