ISSR analysis on genetic diversity of Fagopyrum cymosum collected from eight wild populations.

Objective To discuss the genetic diversity of Fagopyrum cymosum.Methods The genetic diversity of 92 individuals from eight populations were analyzed by ISSR marker.Results Twelve primers were selected to produce highly reproducible ISSR bands.Among 103 amplified bands,90 showed polymorphism,the percentage of polymorphic bands reached 87.38%.Nei′s gene diversity index(H) was 0.374 7,Shannon information index(I) was 0.572 8,Gst was 0.171 8.The genetic distance coefficient and the genetic similarity were 0.043 1～0.384 9 and 0.684 3～0.954 5,respectively.ConclusionF.cymosum has high genetic diversity and the majority of genetic variation occur in the populations.By cluster analysis,the geographical distribution is very obvious.The ISSR marker can be used for the analysis of the genetic diversity and genetic variation of F.cymosum.