Blue-green algae integrative shuttle expression vector and construction method thereof

The invention relates to a blue-green algae integrative shuttle expression vector and a construction method thereof, which comprise an integrative section F1, a blue-green algae promoter PpsbAl, a screen marker gene nptII and an integrative section F2, the vector sequence from the direction of the upstream to the downstream comprises the integrative section F1, the blue-green algae promoter PpsbAl, the screen marker gene nptII and the integrative section F2 in sequence, and the specific sequence refers to the sequence table. The pAnFP integrative shuttle expression vector can be directly recombined with the blue-green algae chromosomal DNA homological locus, an exogenous gene is positioned and integrated on the chromosomal DNA, can be stably expressed in the blue-green algae cell, so the plasmid replication loss phenomenon is avoided.