Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker

2012 
The invention discloses a flow injection chemiluminiscence detection sensor for a tumor marker. A preparation method of the flow injection chemiluminiscence detection sensor comprises the following steps of: preparing a flow injection chemiluminescence flow-through cell by utilizing polymethyl methacrylate; preparing carbon qunatum points, ferroferric oxide coated with carbon, silicon dioxide and hollow gold nano particles according to the existing method; coating the carbon qunatum points on the surfaces of magnetic particles; modifying primary antibodies on the magnetic particles coated with the carbon qunatum points; modifying the hollow gold nano particles on the silicon dioxide; coating secondary antibodies on the hollow gold nano particles positioned on the surface of the silicon dioxide; and carrying out chemiluminescence detection by utilizing flow injection. The flow injection chemiluminiscence detection sensor disclosed by the invention has the advantages of high specificity, high sensitivity, easiness for operation and low detection line.
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