Genetic diversity and phylogenetic relationship analysis on germplasm resources of lily by RAPD.

In this study,the genetic diversity and phylogenetic relationship among 16 genotypes of Lily collected from different ecological sources was analyzed by RAPD. Thirty-five available RAPD primers were screened from 120 random primers,and a total of 769 DNA bands were amplified,in which 628 bands were polymorphic,the percentage is 81.7%. Cluster analysis showed that the 16 Lily germplasms were divided into two groups at 0.9407. Corresponding to two genus in liliaceae:Lilium and Cardiocrinum,and that germplasms in Lilium were further divided into three different types at 0.5790:Lilium brownii F.E. Brown var.viridulum Baker,L. lancifolium Thunb and L. pumilum DC. Results suggested that the genetic diversity is very high among different Lily germplasms and the genetic distance among germplasms was relative to their geographic distance.