Comparative characteristics of antigenic immunosorbents for isolating antibodies to LDH5

: The authors compare the methods for immobilization of porcine lactate dehydrogenase-5 (LDH5) on various carriers to be used for the preparation of an effective immunosorbent for the isolation of monospecific antibodies. Covalent binding of the enzyme with the carrier via a spacer grouping with the use of glutaraldehyde is advisable for LDH M4-isoform that is characterized by a quaternary structure.