Mixed fermentation feed and preparation method and application method of mixed fermentation feed

The invention discloses fermentation feed produced by mixed bacteria such as lactobacillus paracasei, and a preparation method and an application method of the fermentation feed. The lactobacillus paracasei (CICC20296), bacillus subtilis (ACCC10619) and saccharomyces cerevisiae (CICC32236) are subjected to independent liquid fermentation and culture, fermentation liquids are mixed at a volume ratio of 1:1:1, inoculated to a solid culture medium for uniform mixing, and placed in a sealed container for anaerobic fermentation and culture for 75-240h at 10-37 DEG C. The viable count of the feed reaches 200-300*10 cfu*g , and the feed is not required to be dried and can be used directly. According to the preparation method of the fermentation feed, the use amount of antibiotics is reduced, the obtained fermentation feed contains a large number of nutrient substances such as mycoprotein, rich amino acid and vitamins, and facilitates promoting balance of intestinal flora, improving immunity of livestock organisms, improving the production performance and lowering raising cost.