Effects of different pretreatment methods on the structural characteristics, enzymatic saccharification and photo-fermentative bio-hydrogen production performance of corn straw

Abstract In this study, the effects of different pretreatment methods, including hydrothermal, acid, alkali, acid-heat, and alkali-heat on the structural characteristics, enzymatic saccharification and photo-fermentative bio-hydrogen production performance of corn straw were investigated. Results revealed that all the studied pretreatments effectively destroyed the corn straw structure and improved its enzymatic saccharification potential. The alkali-heat and alkali pretreatment showed significant advantage in reducing sugars release, and the highest total reducing sugar concentration of 23.07 g/L was obtained under the pretreatment condition of 2% NaOH-Heat. The maximum cumulative hydrogen yield of 137.76 mL/g TS was achieved from 2% NaOH pretreated corn straw, while corn straw pretreated with 4% NaOH-heat had the minimum cumulative hydrogen yield of 44.20 mL/g TS. These results suggest that appropriate pretreatment can effectively destroy the corn straw structure and enhance its enzymatic saccharification and hydrogen production performance.