[Adenoviral-mediated APE/Ref-1 expression protects rat spiral ganglion cells from oxidative damage].

Objective To address the question if apurinic/apyrimidimic endonuclase/redox factor 1 （APE/Ref-1） involved in preventing spiral ganglion cells oxidative damage after oxidative stress. Methods Primary cultured rat spiral ganglion cells were infected with the adenovirus containing APE/Ref-1 for 48 h, then treated with H2O2 （ 0, 10,25,50,100,300 μmol/L） for 1 h, and finally changed back into normal medium. Western blot were used to detect the level of APE/Ref-1 protein in the infected cells to ensure APE/Ref-1 over expression as a result of adenovirus infection. The cell viability was determined by MTT and the apoptosis of spiral ganglion cells was determined by terminal-deoxynucleotidyl transferase mediated nick end labeling （TUNEL）. Results Western blot showed that infection of adenovirus resulted in APE/Ref-1 over expression in the spiral ganglion cells. Over expression of APE/Ref-1 significantly improved cell viability in cultures treated with different concentration H2O2 from 50 to 300 μmol/L. However, the apoptosis of cells was significantly inhibited. Conclusions Overe xpression of APE/Ref-1 could protect spiral ganglion cells from oxidative damage. Key words: Spiral ganglion; DNA-（apurinic or apyrlmidinie site） lyase; Oxidative stress; Apoptosis