EVALUATION OF IL-1Β SECRETION LEVEL BY HUMAN OSTEOBLAST CELLS ADJUCENT TO WHITE MTA, DARK MTA, PORTLAND CEMENT & IRM AS A RETROGRADE FILLING MATERIAL
2007
Introduction: Ostetoblasts and ligament periodontal cells are the essential cells for wound repair after root-end
resections and perforation repair. Osteoblast cells reaction in direct contact with filling materials play a critical role in
wound repair after surgeries and perforation corrections. Cell attachment on material surfaces and cell secretory
function is the primary phase for evaluation of normal cell function. The aim of this study was to morphologically
evaluate osteoblast cells (MG-63) function and IL-1β secretory level adjacent to gray MTA, white MTA, Portland
cement and IRM, as materials for root-end fillings and repairing perforations.
Materials & Methods: Human osteoblast cells (MG-63) obtained from Iran Pasteur Institue. cell bank were grown
in RPMI-1640 medium. The under study materials, following the company instructions, were mixed and seeded in 24,
1cmx1cm partition plates with the aproximate thickness of 1mm. The cells were added after the materials primary
setting time. The cells were observed by a light microscope an day 1, 3 and 7. In the mentioned intervals, the cells
supernants were collected and examined by ELISA and the amount of IL-1β in each specimen was measured in
pg/ml. The turkey test was used for comparison of the data among different matreials and the Komogrov-Smirnov test
was used for normalizing the resonses.
Results: The cells morphological outcomes illustrated that after 7 days, a large amount of osteoblasts adjacent to
gray and white MTA had the good attachment and morpholigically expansion and flat. The cells adjacent to Portland
cement were found round and mostly seperated from the surface, although some flat cells could be found among
them. Adjacent to IRM, all the cells were round and seperated from the plate surface. The level amount of IL-1β
secretion adjacent to gray and white MTA was significantly more than to IRM and Portland cement (P=0.00).
Adjacent to gray and white MTA, the amount of IL-1β secretion was not significantly different (P=0.77), also the IL-1β
secretion level adjacent to Portland cement and IRM was not significantly different (P=0.187).
Conclusion: The currrent study result indicates that human osteoblasts adjacent to gray MTA and white MTA, in
comparison to Portland cement and IRM, showed a more appropriate response. Therefore we recommed the use of
MTA over the other materials. Regarding Portland cement more research needs to be done in order to reach a final
conclusion.
Key words: Human osteoblasts, retrograde, inter leukine, MTA (mioneral trioxide aggregate).
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI