Neural and Behavioral Responses to Tryptophan Depletion in Unmedicated Patients With Remitted Major Depressive Disorder and Controls

Context An instructive paradigm for investigating the relationship between brain serotonin function and major depressive disorder (MDD) is the response to tryptophan depletion (TD) induced by oral loading with all essential amino acids except the serotonin precursor tryptophan. Objective To determine whether serotonin dysfunction represents a trait abnormality in MDD in the context of specific neural circuitry abnormalities involved in the pathogenesis of MDD. Design Randomized double-blind crossover study. Setting Outpatient clinic. Participants Twenty-seven medication-free patients with remitted MDD (18 women and 9 men; mean ± SD age, 39.8 ± 12.7 years) and 19 controls (10 women and 9 men; mean ± SD age, 34.4 ± 11.5 years). Interventions We induced TD by administering capsules containing an amino acid mixture without tryptophan. Sham depletion used identical capsules containing hydrous lactose. Fluorodeoxyglucose F 18 positron emission tomography studies were performed 6 hours after TD. Magnetic resonance images were obtained for all participants. Main Outcome Measures Quantitative positron emission tomography of regional cerebral glucose utilization to study the neural effects of sham depletion and TD. Behavioral assessments used a modified (24-item) version of the Hamilton Depression Rating Scale. Results Tryptophan depletion induced a transient return of depressive symptoms in patients with remitted MDD but not in controls ( P Conclusion The pattern of TD-induced regional cerebral glucose utilization changes in patients with remitted MDD suggests that TD unmasks a disease-specific, serotonin system–related trait dysfunction and identifies a circuit that probably plays a key role in the pathogenesis of MDD.