Study on the Effect of Medium on Induction and Differentiation of Callus of Angelica sinensis

The seeds of Angelica sinensis are used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The result shows that the optimal madium for callus induction is H+0.5 mg/L 2,4-D,and on it,the callus forming rate could reach to 97.92%;the optimal medium for induction of adventitious buds is 1/2 MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L ZT,and the highest induction rate is 90.3%;1/2 MS+0.5 mg/L IAA is the best for rooting,and the rooting rate could reach to 35.5%.