The Expression of ET-3 and GFAP in the Layers III-VI of Frontal and Parietal Lobes of Mice Following Cerebral Ischemia/Reperfusion

Objective To explore the relationship between ischemia/reperfusion (IR) and the expression ET-3 and GFAP. Methods Thirty-six adult male mice were randomly divided into three groups: ⑴ ischemia/reperfusion group(IR,n=24), bilateral common carotid arteries(BCCA) of mice were ligated for 7 minutes and reperfused for 1day,3day,5day and 10day; ⑵ a sham operation group (SO, n=6); ⑶ normal control group(NG, n=6). The ET-3 and GFAP expressions in the layers Ⅲ-Ⅵ of frontal and parietal lobes of mice in the three groups was detected by immunohistochemisty. Results The expressions of ET-3 and GFAP in the layers Ⅲ-Ⅵ of frontal and parietal lobes significantly increased in I/R group(P0 01) compared with control group, and the maximum expressions of ET-3 and GFAP were observed 5d after reperfusion. Conclusions The expressions of ET-3 and GFAP was up-regulated after IR, which may associate with the damage of central nerve system during I/R.