Induction of binucleation in human lymphocytes by 14 synthetic isoindolone derivatives related to cytochalasins

Abstract Fourteen hydrogenated isoindolone derivatives with affinity to cytochalasin B were synthesized and tested for the induction of binucleation in the human lymphocyte micronucleus (MN) assay. The experimental procedure was the one commonly used for the human lymphocyte MN assay. Compounds like cyt-B were added to cultures at 44 h and tested at increasing concentrations, up to 200 μmol/l, in the range commonly used for cyt-B in the MN assay (3 μ g/ml = 6.25 μ mol/l) Induction of cytokinesis-blocked binucleated cells was found for all compounds but, at the same molarity, cyt-B induced a higher percentage of binucleation. Only one of the compounds tested was found to induce micronuclei significantly: MN were induced across the dose range of 25–100 μmol/l.