Identification of BCAP, a new protein associated with basal bodies and centrioles.

1. Abstract2. Introduction3. Materials and methods3.1. Air-liquid interface (ALI) culture and suspension culture3.2. Antibodies3.3. cDNA differential display3.4. Northern blotting3.5. RT-PCR3.6. Protein analyses3.7. Immunofluorescence4. Results4.1. Identification of genes upregulated during ciliated cell differentiation4.2. The BCAP sequence predicts more than one protein4.3. The pattern of BCAP expression is tissue-specific4.4. BCAP expression is correlated with mucociliary differentiation4.5. BCAP is enriched in centriole/basal bodies of HNE ciliated cells and within centrioles of proliferating cells5. Discussion6. Acknowledgments7. References