三波长-分光光度法测定山楂叶、果总黄酮的含量

In this study, a three-wavelength spectrophotometry method, could eliminate the absorbance error of interfering components, turbid solution and the scattering effect, was developed to determination of flavonoids in hawthorn leaves and fruits. Back - ground changed with concentration changed and asymmetric absorption peak problems can be solved also. The regression equation of concentration △A was obtained and listed as follows: △A=88.795C-0.2060 with a relation coefficient), γ=0. 9994. The experimental results demonstrated the total flavonoids concentrations in 0~40. 66μtg/mL. with △A obeying linear relation, when the absorbance was measured at wavelength λ1= 463nm .λ2=417 nm and λ3 = 382 nm. The recovery is 96.86%~102.46% and the coefficient of variation is 0. 136%. The method exhibited more advantages over the traditional spectrophotometry method.