Nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes

2010 
The invention discloses a nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes, belonging to the field of biological assay. In the method, by virtue of a loop-mediated isothermal amplification (LAMP) technology and a specific primer, the specific area of target genes is amplified by an LAMP technical platform; and with the aid of a positive and negative quality control system and an internal control detection system, nucleic acid screening or detection is carried out on the staphylococcus aureus, the salmonella, the shigella and the listeria monocytogenes from the molecular level. The nucleic acid screening method of the invention has the characteristics of being simple, convenient, economical, fast, sensitive and specific, and the method has wide application prospect.
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