Comparative study on cellulose hydrolysis and acidification in different pathways.

Complicated structure of crops straw may arouse uncorresponding acidification result with traditional sugar crops,To study the acidification effect of lignocellulosic materials conveniently,filter paper which is relatively simple was selected as raw material,to investigate the effects of activity of cellulose,reaction time and ratio of cellulose to yeast inoculation(F/M)on production of ethanol and acetic acid which were produced from cellulose after the joint action of cellulose and yeast as well as anaerobic fermentation process.Results indicated that the maximum glucose production was 73.7 mg/g(24.9% of transformation ratio,TR)in the condition of enzymatic concentration 120 U/g,50 ℃,pH 4.8 and 24 h of hydrolysis.The maximum ethanol production was 119.3 mg/g(42% of TR)in the condition of F/M ratio of 2:1,reaction time 96 h in separate hydrolysis and fermentation(SHF)process corresponding to 396 mg/g(58.2% of TR)in the condition of F/M ratio of 1:2,reaction time 120 h in simultaneous saccharification and fermentation(SSF)process.Ethanol production was enhanced by 34.91% through SSF process than SHF in the condition of F/M ratio of 2:1 and reaction time120 h.