Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor

The invention discloses a preparation method of an unmarked electrochemiluminescent tumor marker immunosensor and application of the immunosensor in detection of tumor markers, and belongs to the field of novel nano functional materials and biosensors. According to the preparation method of the unmarked electrochemiluminescent tumor marker immunosensor, a novel micro-nano material NH4CoPO4 is taken as a substrate so that the luminescence stability of a luminol-hydrogen peroxide luminescent system can be greatly enhanced, and meanwhile, rod-like gold@siver core-shell nanoparticles are taken as a biomimetic catalytic luminescent system, and therefore, an unmarked electrochemiluminescent immunosensor, which is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare and is used for detecting tumor makers, can be prepared.