THE CHLOROPHYLL AND ANTHOCYANIN CONTENT OF REGENERATED POTATO PLANTS OBTAINED FROM PVX AND PVY INFECTED PLANTLETS TREATED BY COMBINED THERAPIES (PRELIMINARY STUDIES)

The purpose of this study was to estimate the effects of combined therapies (electrotherapy and treatments with Satureja hortensis essential oils, H2O2 1mM pH 5.6 applied by spraying the plants acclimated in green house for decrease PVX and PVY infection level. Electrotherapy was applied in 4 variants: after washing and sizing explants, potato stems infected were exposed to 100 and 50 miliampers, for either 10 or 20 minutes, followed by sterilization and planting the axillary buds tip in vitro. Only the healthy plants (obtained from PVY and PVX infected plantlets variety Roclas) were used for the experiment. Monitoring the vegetative state of regenerated plant was done by estimation the chlorophyll (portable device SPAD 502 Chlorophyll Meter) and the anthocyanin (portable device ACM 200 plus, Antocianin Chlorophyll Meter) content of the leaf. Within the decrease of PVY and PVX infection level by using electrotherapy and was noticed a significant decrease of chlorophyll content compared to the negative control in case of variant 100mA/20minutes. Regarding the content of anthocyanin, there were not significant differences between values recorded in the experimental variants. Compared to the negative control, however, it was found small increase of anthocyanin content in case of material initially infected with PVY (but the values were not statistically supported). As opposed the content of anthocyanin, we noticed that monitoring of chlorophyll content indicated beneficial effects of combined therapies over the biological material regenerated from plantlets infected with PVX and PVY and treated.