Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method

: Issledovany otnositel'nye razlichiia posttransliatsionnykh modifikatsiĭ (PTM) belkov v obraztsakh plazmy krovi bol'nykh tserebral'noĭ ishemieĭ (TsI) i zdorovykh liudeĭ na osnove metoda sravnitel'nogo bezmetkovogo proteomnogo analiza na baze tekhnologii tandemnoĭ VEZhKh-MS/MS. Dlia obnaruzheniia PTM byl vypolnen mnozhestvennyĭ poisk MS/MS v baze dannykh Mascot s ustanovlennoĭ peremennoĭ PTM s ispol'zovaniem programmnogo obespecheniia Progenesis LS-MS. V plazme bol'nykh TsI nabliudali uvelichenie doli posttransliatsionno-modifitsirovannykh peptidov. Vyiavleny takie PTM belkov, kak fosforilirovanie ostatkov serina, treonina i tirozina, atsetilirovanie ostatkov lizina i N-kontsevoe atsetilirovanie, ubikvitinirovanie ostatkov lizina i dezamidirovanie ostatkov glutamina i asparagina, imeiushchie otnoshenie k klinicheski znachimym protsessam.