Electrophoretic Analysis of Isoforms of Glyoxalase II in Clinical Blood Samples

A non-denaturing polyacrylamide gel electrophoresis procedure is described for the analysis of isoforms of human red blood cell glyoxalase II (EC 3.1.2.6). A cathodic, continuous buffer electrophoresis system was developed, using a 60 g/1 polyacrylamide gel with 25 g/1 N^-methylene-bis-acrylamide in 50 mmol/1 sodium succinate buffer, pH 5.5 and 1 °C. Staining for glyoxalase II activity involved