Regeneration of Plants by Pollen Culture in Rice (Oryza sativa L.)

Pollen calluses and plants were obtained in rice (Oryza sativa L.) through pollen culture without preculture of anthers. The spikes were pretreated at 10°C for 10-30 days. Isolated pollen grains at the late uninucleate stage were cultured in a medium consisting of one eighth to three eighths of the nutrient constituents of N6 supplemented with 1% sucrose, 1% glucose, 0.1% yeast extract, 0.1% casein hydrolysate, and 10-5M 2, 4-dichlorophenoxyacetic acid. After 11 days of culture, small calluses were induced from the pollen grains. To regenerate plants, viable calluses were transferred from the liquid medium onto the agar media for differentiation. All the regenerated plantlets, including haploid ones, were planted in paddy fields to obtain mature plants.