Construction of a Recombinant Vector Expressing Avian influenza virus Nucleoprotein with an Avian-derived Promoter and Detection of Its Immunogenicity

In order to investigate the immune responses to the nucleoprotein(NP) of Avian influenza virus,NP gene cut from re-combinant plasmid pVAX-NP was subcloned into a eukaryotic expression vector pCAGGS with an avian-derived promoter.The re-combinant plasmid pCAGGS-NP was obtained after PCR identification and sequencing.Then the positive pCAGGS-NP was subject-ed to transfect eukaryocyte and to immunize SPF chickens.Indirect florescent assay(IFA) was engaged to detect the transient expres-sion of NP,and ELISA was used to detect the serum antibody of the immunized chickens which was resistant to NP protein.The re-sults showed that NP was successfully expressed both in vitro and in vivo and had good immunoreactivity.