Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy

Being able to use versatile light microscopy on live or fi xed samples followed by electron microscopy imaging for high resolution analyses is a challenging goal. The advantage is of course that tracing and localizing fl uorescently labeled molecules yields great information about dynamic cellular processes, while electron microscopy of the same sample provides exquisite information about subcellular structures. Here, I describe the straightforward combination of both methods by photoconversion of diaminobenzidine (DAB) through cyan fl uorescent protein (CFP) tagged proteins localized to the Golgi apparatus in primary hippocampal neurons.