Quadruplex Genotyping of TLR-8 Polymorphisms in a Single Reaction

Toll-like Receptor 8 (TLR8) plays an important role in the innate immune defense against various pathogens. The TLR8 genetic variation affects the course of infections with agents such as HIV, HCV, and mycobacteria. To assess the influence of single nucleotide polymorphisms (SNPs) large cohorts need to be studied; thus, rapid and cost-efficient protocols for high-throughput TLR8 genotyping are needed. We designed a single-tube assay for genotyping four SNPs located to the major coding exon of TLR8 using the LightCycler 480 system. The new method is accurate, fast, low-priced, and can be applied for fully automated high-throughput genotyping of TLR8 SNPs.