Ultra-broadband few-cycle laser pulses for advanced multi-color FLIM- microscopy

Multi-photon microscopy has long been a cornerstone in bio-imaging due to its deep-tissue imaging capabilities, reduced scattering and lowered photo-toxicity. For standard femtosecond (fs) laser sources the "normal" experimental strategy to achieve multi-color imaging, is to either employ multiple excitation sources at the same time or to spectrally tune a laser source and to perform multiple scans sequentially, when imaging complex samples labelled with spectrally distinct chromophores.