Determination of Irbesartan in Human Plasma by HPLC
2010
OBJECTIVE To develop a high performance liquid chromatography method for the determination of irbesartan in human plasma.METHODS The plasma were extracted by diethyl ether.The analytical column was packed with ZORBAX Extend-C18.The mobile phase were acetonitrile-water-0.1% trifluoroacetic acid-0.5 mmol.L-1 SDS and the flow rate was 0.8 mL.min-1.The UV detection wavelength was 242 nm(0-6.5min) and 266 nm(6.5-7.8min).RESULTS Excellent liner relationship was obtained in the range of 0.05-6.00 mg.L-1(r=0.999 9).The limit determination of irbesartan was 0.05 mg.L-1.The relative recoveries were(103.93±1.09)%,(99.85±0.65)% and(100.95±1.21)% respectively at three concentrations,the intra-day RSD were 3.04%,1.15% and 1.19% and inter-day RSD were 4.50%,3.32% and 1.18%,respectively.CONCLUSION The method was accurate,simple,rapid and could be used to determine the irbesartan concentration in human plasma and for study of its pharmacokinetics.
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