Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Julia Joung,Silvana Konermann,Jonathan S. Gootenberg,Omar O Abudayyeh,Randall Jeffrey Platt,Brigham,Neville E. Sanjana,Feng Zhang

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

2017

Julia Joung
Silvana Konermann
Jonathan S. Gootenberg
Omar O Abudayyeh
Randall Jeffrey Platt
Brigham
Neville E. Sanjana
Feng Zhang

This protocol from Feng Zhang's lab enables genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system, as sgRNA libraries are constructed and packaged into lentiviral vectors for delivery into cells for screening.

Keywords:

CRISPR
Molecular biology
Genome
Biology
High-throughput screening
Bioinformatics
Phenotype
Genomics
Subgenomic mRNA
genome scale
Genomic library
Gene Knockout Techniques
Genetics

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