Synthesis of Different Degree of Substitution of LAMS and Preliminary Study on Physical and Chemical Properties

Objective: To sulfate the laminarin for different degree of substitution.And its sulfate content,sugar content and molecular testing for the study of different degree of substitution of LAMS lay the material foundation of biological activity.Methods: Using chlorosulfonic acid-pyridine method for sulfation of laminarin,sulfated by changing conditions,preparation of different degree of substitution of sulfate laminarin sulfate;the use of barium sulfate turbidimetric method LAMS of sulfate content,and calculate the degree of substitution;phenol-sulfuric acid method for the determination of laminarin sulfate polysaccharide content and its molecular weight determined using HPGPC.Results: LAMS of two different sulfur degree of substitution of sulfate contents were 37.8%,45.92%,the degree of substitution were 1.07,1.51,sugar contents were 44.52%,37.19%,and molecular weight were 13000,16000.Conclusion: LAMS was prepared from laminarin by chlorsulfonic acid-pyridine method.The rate of esterification are high and preparation of a different degree of substitution of the product by this method.