Clinical significance of pseudogene Cripto-3 mRNA in human colorectal carcinoma

Objective To study the clinical significance of pseudogene Cripto-3 (Cr-3) mRNA in human colorectal carcinoma.Methods After human colorectal normal tissues and colorectal tumoral tissues were collected,the mRNA levels of these tissues were determined by real time polymerase chain reaction.Furthermore,the gender,tumor location,and differentiation degree,and serosal invasion,and lymph node metastasis,and Duke's clinical stage were analyzed.Results The positive rates of Cr-3 in colorectal carcinoma was 75％ (27/36),however,in normal colorectal mucosa,the positive rate of Cr-3 was 0％(0/36) (P ＜ 0.05).The positive rates of Cr-3 was closely correlated to the cancer cell invasion,and lymph node metastasis and Duke' s stage (P ＜ 0.05),but the positive rates of Cr-3 was not correlated to gender,tumor location and differentiation degree(P ＞ 0.05).Conclusion Cr-3 gene might be one of the important target of gene diagnosis and gene therapy of colorectal cancer. Key words: Colon carcinoma;  Cripto-3