Monitoring the kinetics of biocatalytic removal of the endocrine disrupting compound 17α-ethinylestradiol from differently polluted wastewater bodies

Abstract Horseradish peroxidase (HRP)-mediated degradation of 17α-ethinylestradiol (EE2) in artificial wastewater samples was studied qualitatively (HPLC–MS) and quantitatively (HPLC-UV). Experiments were performed with two different HRP isoenzyme variants either of herbal origin (HRPc) or expressed by recombinant yeasts (C1A_6). Experiments were performed under different pH- and salinity conditions, with and without addition of H 2 O 2 at different organic pollution levels mimicked by humic acids. The rapid degradation of the hormone by HRP was demonstrated, and characteristic peaks representing water-soluble degradation products (molar masses 97.2 and 195) were detected and tentatively classified as C5 and C10 compounds. Furthermore, it turned out that H 2 O 2 addition has positive impact on EE2 degradation; especially repeated supplementation of small amounts of H 2 O 2 resulted in complete EE2 degradation within a reasonable time frame for both isoenzyme variants. Increasing humic acid concentration displayed no major inhibition of HRPc’s biocatalytic activity, in contrast to a significant inhibition of variant C1A_6 at increasing humic acid concentrations. Detailed kinetic data were elaborated for the degradation rate of the target substrate EE2; these data shall be used in future to estimate the degradation performance of HRP under operational conditions during wastewater treatment, e.g., when used as enzyme immobilized on hollow fiber membrane modules.